Lower Hgb indicates care during collection and serum processing. Higher Hgb may indicate extended time before separation of blood to serum or cell lysis due to freezing prior to processing. Higher Hgb indicates red and white blood cell lysis and may result in virus release. Three hemoglobin types oxy, met and carboxy.
| Reference | Title | Description |
|---|---|---|
| Fleming | A spectrophotometric method for the quantitative estimation of bilirubin in liquor amnii | Fleming AF, Woolf AJ Clnica chimica act; international j. of clinical chem. 12:1 (1965) pg 67-74 Describes method of spectrophotometric analysis of serum for hemoglobin. Measures oxy-hemoglobin. Abs576x115 – Abs623x102 – Abs700x39.1 = mg/dL |
| USP <90> | Fetal Bovine Serum-Quality Attributes and Functionality Test | Calculate concentration of hemoglobin in mg/dL by absorbance of the serum sample. Abs576x115 – Abs623x102 – Abs700x39.1 = mg/dL |
| Three Wavelength | Direct Spectrophotometry of Serum Hemoglobin: An Allen correction compared with a three-Wavelength Polychromatic Analysis | Hunter F, Morton G, Soutter L (1950) Am. J. of Clin Path 20(5):429-433 Estimates hemoglobin concentration by spectrometry. Polychromatic formula gives concentration estimates approximately 5% greater than those of the Allen correction. Abs415x1.68 – Ab380x0.84 – Abs450x0.84 mA = mg/L |
| Three Wavelength Allen Correction | A spectrophotometric method for quantitating hemoglobin in plasma or serum | Noe D, Weedn V, Bell W; Clin Chem (1984) May;30(5):627-30 Estimates hemoglobin concentration by spectrometry. Abs415x1.65 – Ab380x0.93 – Abs470x0.0.73 mA = mg/L |
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