Relative functionality of a lot to a control serum. Standard performance test, results may vary depending on methodology and other variables. Cell survival in serum to a control. Useful to assess long-term culture of cells to determine any toxic effect present in serum.
| Reference | Title | Description |
|---|---|---|
| USP <90> | Serum Functionality Test | Determine the functionality of specific lots of serum. Growth kinetics is measured by counting viable cells after 7 days of culture on three cell lines. Viable cell counts are determined on days 0, 1, 2, 4, and 7 for cell lag, log and stationary phase. Doubling time of the test sample should be no less than 90% of the doubling time of control. |
| DNA Synthesis | Growth Promotion DNA synthesis | Measures of synthesized DNA in the presence of a label. Proliferating cells contain more DNA than necrotic or apoptotic cells. |
| Metabolic | Growth Promotion Metabolic Activity | Measures metabolic activity of proliferating cells. |
| USP <90> | Cloning | Plating efficiency or colony formation at low cell density evaluated proliferative capacity and survival of single cells under assay growth conditions. Percent plating efficiency is expressed by counting the number of colonies in a defined area divided by the number of cells seeded, multiplied by 100. |
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