Protein – Total

Characteristic of bovine animal age. FBS has the lowest protein concentration of the serum types.

Reference	Title	Description
Biuret	Biuret Method for the Determination of Total Protein in Serum ad Exudates.	Tietz, Norbert W.: Fund. Of Clin. Chem (1976) 302-304 Biuret method is based on the complexation of Cu2+ to functional groups in the protein’s peptide bonds. Produces a violet-colored chelated product when the formation of Cu+2 of two peptide bonds occurs. Intensity of color is directly proportional to protein content.
BCA protein	Measurement of protein using bicinchoninic acid	Smith et.al, Anal Biochemck (1987) May15; 163(1):279 Capable of monitoring cuprious ion produced in the reaction of protein with alkaline Cu2+. Forms a deep purple complex with bicinchonic acid. Measures total protein concertation compared to a protein standard.
Chem Analyzer	Clinical Chemistry Analyzer	Colorimetirc (Biuret). Total protein is measured in g/dL. Interferences include Lipemia>1000, Hemolysis>650 Icterus>21