Characteristic of bovine animal age. FBS has the lowest protein concentration of the serum types.
|Biuret||Biuret Method for the Determination of Total Protein in Serum ad Exudates.||Tietz, Norbert W.: Fund. Of Clin. Chem (1976) 302-304
Biuret method is based on the complexation of Cu2+ to functional groups in the protein’s peptide bonds. Produces a violet-colored chelated product when the formation of Cu+2 of two peptide bonds occurs. Intensity of color is directly proportional to protein content.
|BCA protein||Measurement of protein using bicinchoninic acid||Smith et.al, Anal Biochemck (1987) May15; 163(1):279
Capable of monitoring cuprious ion produced in the reaction of protein with alkaline Cu2+. Forms a deep purple complex with bicinchonic acid. Measures total protein concertation compared to a protein standard.
|Chem Analyzer||Clinical Chemistry Analyzer||Colorimetirc (Biuret). Total protein is measured in g/dL. Interferences include Lipemia>1000, Hemolysis>650 Icterus>21|