## INTRODUCTION

Osmotic pressure plays a critical role in all biological processes that involve diffusion of solutes or transfer of fluids through membranes. Osmosis occurs when solvent but not solute molecules cross a semipermeable membrane from regions of lower to higher concentrations to produce equilibrium. The knowledge of osmotic pressures is important for practitioners in determining whether a parenteral solution is hypo-osmotic, iso-osmotic, or hyperosmotic. A quantitative measure of osmotic pressure facilitates the dilution required to render a solution iso-osmotic relative to whole blood.

## OSMOTIC PRESSURE

The osmotic pressure of a solution depends on the number of particles in solution, and is therefore referred to as a colligative property. A particle can be a molecule or an ion or an aggregated species (e.g., a dimer) that can exist discretely in solution. A solution exhibits ideal behavior when no interaction occurs between solutes and solvent, except where solvent molecules are bound to solutes by hydrogen bonding or coordinate covalency. For such a solution containing a nondissociating solute, the osmotic pressure (𝛑) is directly proportional to its molality (number of moles of solute per kilogram of the solvent):

𝛑= (**ρ***RT*/1000)*m*,

where is the density of the solvent at the temperature *T* (in the absolute scale); R is the universal gas constant; and m is

the molality of the solution. For a real solution containing more than one solute, the osmotic pressure is given by the formula:

𝛑= (**ρ***RT*/1000)Σ^{ν}* _{i}m_{i}*Φ

_{mi,}

where ^{ν}* _{i}* is the number of particles formed by the dissociation of one molecule of the

*i*th solute;

^{ν}

*= 1 for nonionic*

_{i}(nondissociating) solutes;

*m*is the molality of the

_{i}*i*th solute; and Φ

_{mi}is the molal osmotic coefficient of the

*i*th solute. The molal osmotic coefficient takes into account the deviation of a solution from ideal behavior. Its value depends upon the concentration of the solute(s) in solution, its chemical properties, and ionic characteristics. The value of the molal osmotic coefficient of a solute can be determined experimentally by measuring the freezing point depression at different molal concentrations. At concentrations of pharmaceutical interest, the value of the molal osmotic coefficient is less than one. The molal osmotic coefficient decreases with the increase in concentration of the solute (

**).**

*Table 1*## OSMOLALITY

The osmolality of a solution **ξ _{m}** is given by

**ξ _{m}** = Σ

^{ν}

*Φ*

_{i}m_{i}_{m,}

The osmolality of a real solution corresponds to the molality of an ideal solution containing nondissociating solutes and is expressed in osmoles or milliosmoles per kilogram of solvent (Osmol per kg or mOsmol per kg, respectively), a unit that is similar to the molality of the solution. Thus, osmolality is a measure of the osmotic pressure exerted by a real solution across a semipermeable membrane. Like osmotic pressure, other colligative properties of a solution, such as vapor pressure lowering, boiling point elevation, and freezing point depression, are also directly related to the osmolality of the solution. Indeed, the osmolality of a solution is typically determined most accurately and conveniently by measuring freezing point depression (DTf):

**Δ T_{f}=k_{f}ξ_{m},**

where **k_{f}** is the molal cryoscopic constant, which is a property of the solvent. For water, the value of

**is 1.860 per Osmol. That is, 1 Osmol of a solute added to 1 kg of water lowers the freezing point by 1.860 .**

*k*_{f}## OSMOLARITY

Osmolarity of a solution is a theoretical quantity expressed in osmoles per L (Osmol per L) of a solution and is widely used in clinical practice because it expresses osmoles as a function of volume. Osmolarity cannot be measured but is calculated theoretically from the experimentally measured value of osmolality.

Sometimes, osmolarity ( c) is calculated theoretically from the molar concentrations:

ξ_{c}=Σ^{ν}* _{i}c_{i}*,

where ^{ν}* _{i}* is as defined above, and

*c*is the molar concentration of the

_{i}*i*th solute in solution. For example, the osmolarity of a solution prepared by dissolving 1 g of vancomycin in 100 mL of 0.9% sodium chloride solution can be calculated as follows:

The results suggest that the solution is slightly hyperosmotic since the osmolality of blood ranges between 285 and 310 mOsmol per kg. However, the solution is found to be hypo-osmotic and has an experimentally determined osmolality of 255 mOsmol per kg.^{1} The example illustrates that osmolarity values calculated theoretically from the concentration of a solution should be interpreted cautiously and may not represent the osmotic properties of infusion solutions.

The discrepancy between theoretical (osmolarity) and experimental (osmolality) results is, in part, due to the fact that osmotic pressure is related to osmolality and not osmolarity. More significantly, the discrepancy between experimental results and the theoretical calculation is due to the fact that the osmotic pressure of a real solution is less than that of an ideal solution because of interactions between solute molecules or between solute and solvent molecules in a solution. Such interactions reduce the pressure exerted by solute molecules on a semipermeable membrane, reducing experimental values of osmolality compared to theoretical values. This difference is related to the molal osmotic coefficient (Φ* _{m,i}*). The example also illustrates the importance of determining the osmolality of a solution experimentally, rather than calculating the value theoretically.

## MEASUREMENT OF OSMOLALITY

The osmolality of a solution is commonly determined by the measurement of the freezing point depression of the solution.

Apparatus— The apparatus, an osmometer for freezing point depression measurement, consists of the following: a means of cooling the container used for the measurement; a resistor sensitive to temperature (thermistor), with an appropriate currentor potential-difference measurement device that may be graduated in temperature change or in osmolality; and a means of mixing the sample.

Osmometers that measure the vapor pressures of solutions are less frequently employed. They require a smaller volume of specimen (generally about 5 μL), but the accuracy and precision of the resulting osmolality determination are comparable to those obtained by the use of osmometers that depend upon the observed freezing points of solutions.

Standard Solutions— Prepare Standard Solutions as specified in Table 1, as necessary.

Test Solution— For a solid for injection, constitute with the appropriate diluent as specified in the instructions on the labeling. For solutions, use the sample as is. [NOTE—A solution can be diluted to bring it within the range of measurement of the osmometer, if necessary, but the results must be expressed as that of the diluted solution and must NOT be multiplied by a dilution factor to calculate the osmolality of the original solution. The molal osmotic coefficient is a function of concentration. Therefore, it changes with dilution.]

Procedure— Set the zero of the apparatus using water. To calibrate the apparatus, choose at least two solutions from Table 1 such that the osmolalities of the Standard Solutions span the expected range of osmolality of the Test Solution. Introduce an appropriate volume of each Standard Solution into the measurement cell as per the manufacturer’s instructions, and start the cooling system. Usually, the mixing device is programmed to operate at a temperature below the lowest temperature expected from the freezing point depression. The apparatus indicates when the equilibrium is attained. Calibrate the osmometer using an appropriate adjustment device such that the reading corresponds to either the osmolality or freezing point depression value of the Standard Solution shown in Table 1. [NOTE—Some instruments indicate osmolality and some others show freezing point depression.] Before each measurement, rinse the measurement cell at least twice with the solution to be tested. Repeat the procedure with each Test Solution. Read the osmolality of the Test Solution directly, or calculate it from the measured freezing point depression.

Assuming that the value of the osmotic coefficient is essentially the same whether the concentration is expressed in molality or molarity, the experimentally determined osmolality of a solution can be converted to osmolarity in the same manner in which the concentration of a solution is converted from molality to molarity. Unless a solution is very concentrated, the osmolarity of a solution ( c) can be calculated from its experimentally determined osmolality (ξ_{c}):

ξ_{c} = 1000ξ_{c} / (1000 /**ρ**+Σ*w _{i}^{v}_{i}*),

where *w _{i}* is the weight in g; and

*is the partial specific volume, in mL per g, of the*

^{v}_{i}*i*th solute. The partial specific volume of a solute is the change in volume of a solution when an additional 1 g of solute is dissolved in the solution. This volume can be determined by the measurement of densities of the solution before and after the addition of the solute. The partial specific volumes of salts are generally very small, around 0.1 mL per g. However, those of other solutes are generally higher. For example, the partial specific volumes of amino acids are in the range of 0.6–0.9 mL per g.

^{1 }Kastango, E.S. and Hadaway, L. International Journal of Pharmaceutical Compounding 5, (2001) 465-469.

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